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JoVE Journal Neuroscience
In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos
 

In Situ Hybridization Combined with Immunohistochemistry in Cryosectioned Zebrafish Embryos

Article DOI: 10.3791/63715-v • 07:36 min • March 3rd, 2022
March 3rd, 2022 •
*1,2, *3, 2, 4, 2, 1, 2,3, 5, 1
1School of Life Sciences, Nantong University, 2Medical School, Nantong University, 3Key Laboratory of Neuroregeneration of Jiangsu Province and Ministry of Education, Co-Innovation Center of Neuroregeneration, Nantong University, 4Nantong Maternal and Child Health Care Hospital, 5Laboratory Animals Center, Nantong University
* These authors contributed equally

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This protocol describes how to obtain images by combining in situ hybridization and immunohistochemistry of zebrafish embryonic sections. In situ hybridization was performed prior to cryosectioning, followed by antibody staining. It is useful to detect the expression patterns of two genes in zebrafish if there is a paucity of antibodies.

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Keywords: In Situ Hybridization Immunohistochemistry Cryosectioned Zebrafish Embryos MRNA Protein Analysis Fixation Dehydration Rehydration Proteinase K Digestion Pre-hybridization Hybridization Probe Washing SSCT SSC
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