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Small-Scale Extraction of Caenorhabditis elegans Genomic DNA
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Journal JoVE Biologie
Small-Scale Extraction of Caenorhabditis elegans Genomic DNA

Small-Scale Extraction of Caenorhabditis elegans Genomic DNA

DOI:
10.3791/63716-v

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06:40 min

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June 07, 2022

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, ,
1Department of Biology,Texas Woman’s University

Chapitres

  • 00:04Introduction
  • 00:51Single-Worm DNA Extraction
  • 02:54DNA Extraction of a Few Individuals
  • 04:33Results: Successful Use of the Extracted Caenorhabditis elegans DNA as PCR Template
  • 05:44Conclusion

Summary

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Traduction automatique

Presented here is a description of the straightforward and relatively rapid isolation of Caenorhabditis elegans genomic DNA from one or a few animals using a commercially available tissue kit. The resulting gDNA preparation is a suitable template for PCR.

Tags

Caenorhabditis ElegansGenomic DNA ExtractionSmall-scaleClassroomRecherchePCRLysis ProgramExtraction SolutionNeutralization SolutionL4Adult HermaphroditePlatinum Wire PickCentrifugation

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