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Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins
Journal JoVE
Biochimie
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Journal JoVE Biochimie
Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

Simultaneous Interference Reflection and Total Internal Reflection Fluorescence Microscopy for Imaging Dynamic Microtubules and Associated Proteins

DOI:
10.3791/63730-v

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06:43 min

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May 03, 2022

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, ,
1Department of Molecular Biophysics & Biochemistry,Yale University, 2Department of Physics,Yale University

Chapitres

  • 00:05Introduction
  • 00:29Preparation of PDMS Channels
  • 02:15Preparation for Imaging
  • 03:02Growing Dynamic Guanosine Diphosphate “Extensions”
  • 03:46Kinesin Motility Assay
  • 04:23Image Processing and Analysis
  • 05:22Results: Imaging of Dynamic Microtubules and Fluorescently Labeled Microtubule-Associated Proteins
  • 05:57Conclusion

Summary

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Traduction automatique

We present a protocol for implementing interference-reflection microscopy and total-internal-reflection-fluorescence microscopy for the simultaneous imaging of dynamic microtubules and fluorescently labeled microtubule-associated proteins.

Tags

Keywords: Simultaneous Interference ReflectionTotal Internal Reflection Fluorescence MicroscopyDynamic MicrotubulesAssociated ProteinsLabel-free MicrotubulesFluorescently Labeled ProteinsPDMS PolymerMaster MoldPlasma CleaningMicrochannelsIRM ImagingFluorescent MicrobeadsMicrotubule Extension Mixture

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