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Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture
 

Maintaining Human Glioblastoma Cellular Diversity Ex vivo using Three-Dimensional Organoid Culture

Article DOI: 10.3791/63745-v • 07:11 min • August 25th, 2022
August 25th, 2022 •
1, 2, 1, 2
1Department of Neurological Surgery, Cleveland Clinic, 2Cardiovascular and Metabolic Sciences, Lerner Research Institute, Cleveland Clinic

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Here, we describe a method of generating glioblastoma (GBM) organoids from primary patient specimens or patient-derived cell cultures and maintaining them to maturity. These GBM organoids contain phenotypically diverse cell populations and recreate tumor microenvironments ex vivo.

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Human Glioblastoma Cellular Diversity Ex Vivo Three-dimensional Organoid Culture Treatments Tumors Microenvironments Spatial Diversity Biology Traditional Cell Culture Methods Homogeneous Tumor Cell Populations Molecular Diversity Intratumoral Interactions Drug Efficacy Mechanisms Of Resistance Clinical Therapies Sphere Culture Regional Effects Of Chemotherapies Niche Specific Therapies Personalized Medicine Temperature Sensitive Materials Organoid Molds
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