Effect of Hyaluronic Acid 35 kDa on an <em>In Vitro</em> Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Adam Wilson; Kathryn Burge; Jeffrey Eckert; Hala Chaaban

doi:10.3791/63758

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Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

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Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

DOI:

10.3791/63758-v

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09:36 min

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July 28, 2022

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Adam Wilson*¹, Kathryn Burge*¹, Jeffrey Eckert, Hala Chaaban

¹Department of Pediatrics, Division of Neonatal-Perinatal Medicine,University of Oklahoma Health Sciences Center

Chapitres

00:04Introduction
00:46Enteroid Monolayer Formation
03:47Monolayer Treatment and Scratch Wound Assay
06:36Scratch Wound Analysis
07:38Results: Enteroid Generation and Enteroid-Derived Monolayer Wound Healing Assay Procedure, Images, Analysis, and the Effect of HA35 on Wound Healing
08:58Conclusion

Summary

Traduction automatique

This protocol describes a method to establish and perform a scratch wound assay on two-dimensional (2D) monolayers derived from three-dimensional (3D) enteroids isolated from non-human primate ileum.

Effect of Hyaluronic Acid 35 kDa on an In Vitro Model of Preterm Small Intestinal Injury and Healing Using Enteroid-Derived Monolayers

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