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1Department of Biochemistry, Faculty of Pharmacy,Mahidol University, 2Program in Translational Medicine, Faculty of Medicine Ramathibodi Hospital,Mahidol University, 3Excellent Center for Drug Discovery, Faculty of Science,Mahidol University, 4Department of Biotechnology, Faculty of Science,Mahidol University, 5Department of Pharmacology, Faculty of Medicine Siriraj Hospital,Mahidol University
Chapitres
- 00:05Introduction
- 00:50Measuring the Sodium Taurocholate Co-Transporting Polypeptide (NTCP) Using Flow Cytometry
- 04:30Anti-HBV (Hepatitis B Virus) Entry Assay
- 06:13HBV (Hepatitis B Virus) -Cell Binding Assay
- 06:50Taurocholic Acid Uptake Assay
- 07:34Determination of Protein-Ligand Interactions Using Isothermal Titration Calorimetry
- 09:14Results: Screening of Anti-Hepatitis B Virus Using Isothermal Titration Calorimetry
- 10:36Conclusion
We present a protocol to screen anti-hepatitis B virus (HBV) compounds targeting pre- and post-viral entry lifecycle stages, using isothermal titration calorimetry to measure binding affinity (KD) with host sodium taurocholate cotransporting polypeptide. Antiviral efficacy was determined through the suppression of viral lifecycle markers (cccDNA formation, transcription, and viral assembly).
