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En multiplexed screening med høj kapacitet for type 1-diabetes, cøliaki og COVID-19
Journal JoVE
Immunologie et infection
This content is Free Access.
Journal JoVE Immunologie et infection
A High-Throughput Multiplexed Screening for Type 1 Diabetes, Celiac Diseases, and COVID-19
DOI:

06:46 min

July 05, 2022

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Chapitres

  • 00:04Introduction
  • 01:06Create Linker‐Coupled Antigen Solution
  • 02:36Preparation of Antigen Labeled Serum and the 6‐Plex Plate
  • 03:54Serum/Antigen Incubates Transfer into the 6‐Plex Plate
  • 04:42Washing the 6‐Plex Plate and Reading Buffer Addition
  • 05:11Results: Comparison of Antibody Levels Study Between the Single and the 6‐Plex ECL Assay
  • 05:59Conclusion

Summary

Traduction automatique

I tråd med det presserende behov for screening for type 1-diabetes, cøliaki og coronavirus sygdom 2019 udviklede vi et 6-Plex elektrokemiluminescensassay med høj gennemstrømning for samtidig at detektere alle fire ø-autoantistoffer, vævstransglutaminaseautoantistoffer og antistoffer mod receptorbindingsdomænet for alvorligt akut respiratorisk syndrom coronavirus 2.

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