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Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects
Journal JoVE
Médecine
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Journal JoVE Médecine
Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

Flow Cytometric Analysis of Biomarkers for Detecting Human Sperm Functional Defects

DOI:
10.3791/63790-v

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08:48 min

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April 21, 2022

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, , , , , , , , ,
1Institute of Toxicology, College of Preventive Medicine,Army Medical University (Third Military Medical University), 2Department of Chemical Defense Medicine, College of Military Preventive Medicine,Army Medical University (Third Military Medical University)

Chapitres

  • 00:04Introduction
  • 01:01Cell Preparation and Sperm Cells Counting Using Computer‐Aided Sperm Analysis (CASA)
  • 01:37Staining Sperm Cells
  • 04:52Flow Cytometer Setup
  • 07:10Results: Flow Cytometric Analysis of Sperm Function Markers for Detecting Human Sperm Functional Defect in a Single Cytometry
  • 08:22Conclusion

Summary

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The present protocol provides a practical solution that allows for the measurement of apoptosis, mitochondrial membrane potential, and DNA damage in human sperm with a single cytometer.

Tags

Flow CytometrySperm Functional BiomarkersSperm ApoptosisMitochondrial Membrane PotentialSperm Function AnalysisInfertility DiagnosisSemen AnalysisJC-1 ProbeCCCPAnnexin-V FITC PI Staining

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