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水凝胶阵列可提高 3D 肿瘤模型中基质成分和治疗药物筛选效果的通量

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Bio-ingénierie

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Journal JoVE Bio-ingénierie

Hydrogel Arrays Enable Increased Throughput for Screening Effects of Matrix Components and Therapeutics in 3D Tumor Models

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水凝胶阵列可提高 3D 肿瘤模型中基质成分和治疗药物筛选效果的通量

DOI:

10.3791/63791-v

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10:49 min

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June 16, 2022

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Jesse Liang, Alireza Sohrabi, Mary Epperson, Laila M. Rad, Kelly Tamura, Mayilone Sathialingam, Thamira Skandakumar, Philip Lue, Jeremy Huang, James Popoli, Aidan Yackly, Michael Bick, Ze Zhong Wang, Chia-Chun Chen, Grigor Varuzhanyan, Robert Damoiseaux, Stephanie K. Seidlits

¹University of California Los Angeles

Chapitres

00:05Introduction
00:50Preparation of Hydrogel Solution
02:32Illumination and Photocrosslinking of Hydrogels via an LED Array
04:20Setting Up and Drug Treatment of 3D, Matrix-Embedded Cultures
08:22Results: Effect of HA Based 3D Miniaturized Cultures on Matrix Stiffness and Integrin-Binding Peptides
10:24Conclusion

Summary

Traduction automatique

本方案描述了一个实验平台，用于评估机械和生化线索对3D基质模拟培养物中患者来源的胶质母细胞瘤细胞的化疗反应的影响，使用定制的UV照明装置促进具有可调机械特征的水凝胶的高通量光交联。

Tags

3D Tumor Models Hydrogel Arrays High-throughput Screening Matrix Components Therapeutics GBM Cells Drug Resistance HEPES-buffered Solution Thiolated Hyaluronic Acid PEG Norbornene PEG Thiol Cysteine-thiol-containing Peptides UV LED Illumination

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