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Label-Retention Expansion Microscopy (LR-ExM) Enables Super-Resolution Imaging and High-Efficiency Labeling
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Journal JoVE Biologie
Label-Retention Expansion Microscopy (LR-ExM) Enables Super-Resolution Imaging and High-Efficiency Labeling

Label-Retention Expansion Microscopy (LR-ExM) Enables Super-Resolution Imaging and High-Efficiency Labeling

DOI:
10.3791/63793-v

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07:44 min

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October 11, 2022

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, ,
1Center for Complex Biological Systems,University of California Irvine, 2Department of Developmental and Cell Biology,University of California Irvine, 3Department of Chemistry,University of California Irvine

Chapitres

  • 00:05Introduction
  • 00:58Cell Culture, Fixation, and Permeabilization
  • 01:31Endogenous Streptavidin, Biotin Blocking, Primary Antibody Staining, and LR-ExM Specific Secondary Antibody Staining
  • 02:29Additional Anchoring, Gelation, and Digestion
  • 04:26Post-Digestion Fluorescence Staining, Expansion, and Imaging
  • 05:34Results: Labelling the Target Proteins Using Trifunctional Anchors in Label Retention Expansion Microscopy
  • 07:01Conclusion

Summary

Traduction automatique

Traduction automatique

A protocol of label retention expansion microscopy (LR-ExM) is demonstrated. LR-ExM uses a novel set of trifunctional anchors, which provides better labeling efficiency compared to previously introduced expansion microscopies.

Tags

Keywords: Label-Retention Expansion Microscopy (LR-ExM)Super-resolution ImagingHigh-efficiency LabelingTri-functional AnchorsFluorescence RetentionCell CultureFixationPermeabilizationAntibody LabelingSecondary AntibodyGlutaraldehydeHydrogel AnchoringMonomer SolutionGelation

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