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Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid
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Recherche en cancérologie
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Journal JoVE Recherche en cancérologie
Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid

Production of High-Titer Recombinant Newcastle Disease Virus from Allantoic Fluid

DOI:
10.3791/63817-v

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07:24 min

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May 25, 2022

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, , , , , ,
1Department of Pathobiology,University of Guelph

Chapitres

  • 00:04Introduction
  • 00:55Purification of NDV from Allantoic Fluid
  • 05:08Quality Control Assays
  • 05:55Results: Production of High-Titer Recombinant NDV
  • 06:53Conclusion

Summary

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Traduction automatique

Here we provide a detailed procedure for production, purification, and quantification of high-titer recombinant Newcastle disease virus. This protocol consistently yields > 6 × 109 plaque-forming units/mL, providing virus quantities appropriate for in vivo animal studies. Additional quality control assays to ensure safety in vivo are described.

Tags

Newcastle Disease VirusRecombinant VirusVirus PurificationTangential Flow FiltrationAllantoic FluidDepth FiltrationBuffer ExchangePressure ControlVirus Concentration

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