Journal
/
Biologie
/
Reaktivering av demembranerte cellemodeller i Chlamydomonas reinhardtii
Journal JoVE
Biologie
Author Produced
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Biologie
Reactivation of Demembranated Cell Models in Chlamydomonas reinhardtii

Reaktivering av demembranerte cellemodeller i Chlamydomonas reinhardtii

DOI:
10.3791/63869-v

•

03:37 min

•

May 06, 2022

•

Noriko Ueki1, Atsuko Isu2, Ken‒ichi Wakabayashi2,3
1Science Research Center,Hosei University, 2Laboratory for Chemistry and Life Science, Institute of Innovative Research,Tokyo Institute of Technology, 3School of Life Science and Technology,Tokyo Institute of Technology

Chapitres

  • 00:04Introduction
  • 00:46Preparation of Demembranated Cell Models
  • 01:47Reactivation of Demembranated Cell Models
  • 02:16Results: Demembranation and Reactivation of Chlamydomonas reinhardtii Cell Models
  • 02:56Conclusion

Summary

Traduction automatique

Traduction automatique

In vitro reaktivering av motile celler er et avgjørende eksperiment for å forstå mekanismene for cellemotilitet. Protokollen beskriver reaktivering av de demembranerte cellemodellene til Chlamydomonas reinhardtii, en modellorganisme for å studere cilia/flagella.

Tags

ReactivationDemembranated Cell ModelsChlamydomonas ReinhardtiiCilia MotilityCalcium Ion ConcentrationsIn Vitro ObservationMotile Cilia ResearchCentrifugationWashing BufferDemembranation BufferCell PelletDilution BufferMicroscope ObservationReactivation Solution

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/63869?article_type=v

Mating and Tetrad Separation of Chlamydomonas reinhardtii for Genetic Analysis
check_url/fr/63869?article_type=v

Heterotopic and Orthotopic Tracheal Transplantation in Mice used as Models to Study the Development of Obliterative Airway Disease
check_url/fr/63869?article_type=v

Video Bioinformatics Analysis of Human Embryonic Stem Cell Colony Growth
check_url/fr/63869?article_type=v

Principles of Rodent Surgery for the New Surgeon
check_url/fr/63869?article_type=v

Manuell Restraint og Common Compound Administrasjon Ruter i mus og rotter
check_url/fr/63869?article_type=v

Evaluation of Mammary Gland Development and Function in Mouse Models
check_url/fr/63869?article_type=v

Diagnostic Necropsy and Selected Tissue and Sample Collection in Rats and Mice
check_url/fr/63869?article_type=v

Biochemical Reconstitution of Steroid Receptor•Hsp90 Protein Complexes and Reactivation of Ligand Binding
check_url/fr/63869?article_type=v

Mus Sperm Kryokonservering og Recovery bruke I · Cryo Kit
check_url/fr/63869?article_type=v

Omprogrammering Menneskelige Somatiske celler i Utførte pluripotent stamceller (iPSCs) Bruke retrovirale Vector med GFP

Read Article