Journal
/
Médecine
/
Determining Binding Affinity (KD) of Radiolabeled Antibodies to Immobilized Antigens
Journal JoVE
Médecine
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Médecine
Determining Binding Affinity (KD) of Radiolabeled Antibodies to Immobilized Antigens

Determining Binding Affinity (KD) of Radiolabeled Antibodies to Immobilized Antigens

DOI:
10.3791/63927-v

•

07:39 min

•

June 23, 2022

•

, , ,
1Department of Radiology and Biomedical Imaging,Yale University

Chapitres

  • 00:04Introduction
  • 00:49Buffer Preparation
  • 02:05Antigen Immobilization and Blocking Nonspecific Sites with BSA
  • 03:21Serial Dilutions and Addition of the Radiolabeled Antibody (rAb) Solution
  • 05:08Wash Plates and Assay Radioactivity
  • 06:07Results: Determination of Binding Affinity
  • 07:04Conclusion

Summary

Traduction automatique

Traduction automatique

Here, a method is described to determine the binding affinity (KD) of radiolabeled antibodies to immobilized antigens. KD is the equilibrium dissociation constant that can be determined from a saturation binding experiment by measuring the total, specific, and nonspecific binding of a radiolabeled antibody at various concentrations to its antigen.

Tags

Keywords: Binding AffinityKDRadiolabeled AntibodiesImmobilized AntigensNuclear ImagingNuclear TherapyAntigen ImmobilizationBinding BufferBlocking BufferWashing BufferBSATween 20Serial Dilutions

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/63927?article_type=v

The Measurement and Treatment of Suppression in Amblyopia
check_url/fr/63927?article_type=v

Determining The Electromyographic Fatigue Threshold Following a Single Visit Exercise Test
check_url/fr/63927?article_type=v

Determining Glucose Metabolism Kinetics Using 18F-FDG Micro-PET/CT
check_url/fr/63927?article_type=v

A Clinical Trial Assessing the Safety, Efficacy, and Delivery of Olive-Oil-Based Three-Chamber Bags for Parenteral Nutrition
check_url/fr/63927?article_type=v

Determining and Controlling External Power Output During Regular Handrim Wheelchair Propulsion
check_url/fr/63927?article_type=v

Monitoring PD-1-Blocking Antibodies Bound to T Cells Derived from a Drop of Peripheral Blood
check_url/fr/63927?article_type=v

Biological Preparation and Mechanical Technique for Determining Viscoelastic Properties of Zonular Fibers
check_url/fr/63927?article_type=v

Improved Renal Denervation Mitigated Hypertension Induced by Angiotensin II Infusion
check_url/fr/63927?article_type=v

Network Pharmacology Prediction and Experimental Validation of Trichosanthes–Fritillaria thunbergii Action Mechanism Against Lung Adenocarcinoma
check_url/fr/63927?article_type=v

Understanding the Mechanism of Tuina Manipulation in Rats (Video) | JoVE

Read Article