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Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)
Journal JoVE
Biologie
Author Produced
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Journal JoVE Biologie
Lentiviral Mediated Delivery of shRNAs to hESCs and NPCs Using Low-cost Cationic Polymer Polyethylenimine (PEI)
DOI:

06:49 min

May 24, 2022

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Chapitres

  • 00:04Introduction
  • 00:28Transfection of HEK293T Cells Using Either Polymer Polyethylenimine (PEI) or Lipofectamine 3000 Reagent
  • 02:48Lentiviral Vectors (LVS) Collection and Ultracentrifugation
  • 05:07Results: Lentiviral Mediated Delivery of shRNAs in hESCs and NPCs Using Cationic Polymer PEI
  • 06:19Conclusion

Summary

Traduction automatique

Using the low-cost cationic polymer polyethylenimine (PEI), we produced lentiviral particles for stable expression of shRNAs in H9 human embryonic stem cells (hESCs) and transiently transduced H9-derived neural progenitor cells (NPCs) at high efficiency.

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