Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Department of Obstetrics and Gynecology, Guangdong Provincial Key Laboratory of Major Obstetric Diseases,The Third Affiliated Hospital of Guangzhou Medical University, 2Key Laboratory of Reproduction and Genetics of Guangdong Higher Education Institutes, 3School of Biotechnology and Health Sciences,Wuyi University
Chapitres
- 00:04Introduction
- 00:51Induced Pluripotent Stem Cells (iPSCs) and EB Preparation (Days 0 1)
- 02:54Soft Light Lamp Preparation (Day 1), EB Transfer, and Medium Replacement (Days 2 5)
- 04:24Neural Induction (Days 5 7)
- 05:25Embedding in the Basement Membrane Matrix (Days 7 10)
- 06:52Organoid Maturation (Days 10 40)
- 07:21Results: Analysis of Cerebral Organoids
- 08:41Conclusion
Cerebrale organoider gir enestående muligheter for å studere organutvikling og menneskelig sykdomspatologi. Selv om stor suksess er oppnådd med cerebrale organoidkultursystemer, er det fortsatt operasjonelle vanskeligheter med å anvende denne teknologien. Denne protokollen beskriver en cerebral organoid prosedyre som muliggjør medium endring og organoidoverføring.
