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A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro
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Biochimie
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Journal JoVE Biochimie
A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

A Semi-Automated and Reproducible Biological-Based Method to Quantify Calcium Deposition In Vitro

DOI:
10.3791/64029-v

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11:30 min

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June 02, 2022

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, , , , , ,
1Department of Biochemistry, Cardiovascular Research Institute Maastricht (CARIM),Maastricht University, 2Helmholtz Institute for Biomedical Engineering, Biointerface Group,RWTH Aachen University, 3Institute of Experimental Medicine and Systems Biology,RWTH Aachen University

Chapitres

  • 00:05Introduction
  • 00:36Cell Seeding, Maintenance, and Calcification Induction
  • 02:13Calcification Detection via Imaging
  • 07:51Data Analysis
  • 10:13Results: A Semi-Automated Method for In Vitro Calcification Determination
  • 11:05Conclusion

Summary

Traduction automatique

Traduction automatique

Cardiovascular disease is the leading cause of death worldwide. Vascular calcification contributes substantially to the burden of cardiovascular morbidity and mortality. This protocol describes a simple method to quantify vascular smooth muscle cell-mediated calcium precipitation in vitro by fluorescent imaging.

Tags

CalcificationVascular Smooth Muscle CellsFluorescent-based DetectionHVSMCSCalcification MediumDAPIRFPBright FieldImagingData Reduction

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