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Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a
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Journal JoVE Biologie
Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

Field-Deployable Candidatus Liberibacter asiaticus Detection Using Recombinase Polymerase Amplification Combined with CRISPR-Cas12a

DOI:
10.3791/64070-v

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09:03 min

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December 23, 2022

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, , , , , , ,
1China-USA Citrus Huanglongbing Joint Laboratory, National Navel Orange Engineering Research Center,Gannan Normal University, 2Department of Biological Sciences,Gannan Normal University, 3Citrus Research and Education Center, Department of Microbiology and Cell Science,University of Florida – Institute of Food and Agricultural Sciences

Chapitres

  • 00:05Introduction
  • 01:41Construction of CLas-DETECTR
  • 04:09Specificity Test
  • 05:35Representative Results
  • 08:47Conclusion

Summary

Traduction automatique

Traduction automatique

In this work, a rapid, sensitive, and portable detection method for Candidatus Liberibacter asiaticus based on recombinase polymerase amplification combined with CRISPR-Cas12a was developed.

Tags

Candidatus Liberibacter AsiaticusHLBCLas-DETECTRRecombinase Polymerase AmplificationCRISPR-Cas12aField-deployableRapid DetectionIsothermal AmplificationCitrus DiseasePlant DNA ExtractionFluorescent Visualization

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