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通过全内反射荧光(TIRF)显微镜 在体外 可视化肌动蛋白和微管耦合动力学
Journal JoVE
Biochimie
This content is Free Access.
Journal JoVE Biochimie
Visualizing Actin and Microtubule Coupling Dynamics In Vitro by Total Internal Reflection Fluorescence (TIRF) Microscopy
DOI:

08:44 min

July 20, 2022

Chapitres

  • 00:04Introduction
  • 00:40Coating Cleaned Coverslips with mPEG- and Biotin-PEG-Silane
  • 01:21Assembling Imaging Flow Chambers
  • 02:50Conditioning of Perfusion Chambers
  • 03:47Microscope Preparation
  • 04:40Preparation of Protein Reaction Mixes
  • 06:02Image Actin and Microtubule Dynamics
  • 06:43Results: Example Measurements of Microtubules and Actin Filament Dynamics
  • 08:14Conclusion

Summary

Traduction automatique

该方案是使用 体外 全内部荧光(TIRF)显微镜测定可视化动态肌动蛋白和微管的指南。

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