/

Recherche en cancérologie

/

用于肿瘤球体半高通量分析的快速光学清除

Journal JoVE
Recherche en cancérologie

This content is Free Access.

Journal JoVE Recherche en cancérologie

Rapid Optical Clearing for Semi-High-Throughput Analysis of Tumor Spheroids

Please note that all translations are automatically generated. Click here for the English version.

用于肿瘤球体半高通量分析的快速光学清除

DOI:

10.3791/64103-v

•

08:12 min

•

August 23, 2022

•

Gency Gunasingh, Alexander P. Browning, Nikolas K. Haass

¹The University of Queensland Diamantina Institute,The University of Queensland, ²School of Mathematical Sciences,Queensland University of Technology

Chapitres

00:04Introduciton
00:43Preparation of 2% Agarose‐PBS Gel and Clearing Solution
01:39Spheroid Preparation and Staining
04:04Spheroid Mounting and Imaging
05:46Results: Semi-High‐Throughput Imaging of 3D Tumor Spheroids
07:36Conclusion

Summary

Traduction automatique

肿瘤球体越来越多地用于评估肿瘤细胞 - 微环境相互作用和治疗反应。本方案描述了一种使用快速光学清除对3D肿瘤球体进行半高通量成像的稳健但简单的方法。

Tags

Tumor Spheroids 3D Tumor Modeling Optical Clearing Rapid Clearing Semi-high-throughput Analysis Agarose Overlay Method Paraformaldehyde Fixation Antibody Staining Triton X-100 Permeabilization

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

用触印细胞学方法快速获得临床病理标本中高质量肿瘤 DNA

高通量三维肿瘤球体药物筛选的生成

数字聚合酶链反应法测定零星家族性腺瘤性息肉病患者的基因变异

三维肿瘤球体的纵向形态和生理监测

循环肿瘤细胞的微操作, 用于下游分子分析和转移电位评估

小肠神经内分泌肿瘤类球体的建立与特征

固体肿瘤细胞系侧面人口分析

斑马鱼拉瓦尔异种草和肿瘤行为分析的一代

Exploring Mitochondrial Energy Metabolism of Single 3D Microtissue Spheroids Using Extracellular Flux Analysis

工业化、人工智能引导的激光显微切割，用于肿瘤微环境的微尺度蛋白质组学分析

Read Article