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Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice
Journal JoVE
Neurosciences
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Journal JoVE Neurosciences
Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

Simultaneous Imaging of Microglial Dynamics and Neuronal Activity in Awake Mice

DOI:
10.3791/64111-v

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08:26 min

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August 23, 2022

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, , , ,
1Department of Cellular Neurobiology, Graduate School of Medicine and Faculty of Medicine,The University of Tokyo

Chapitres

  • 00:04Introduction
  • 01:00AAV Injection
  • 03:04Cranial Window Implantation
  • 04:36In Vivo Two‐Photon Imaging of Microglia and Neuronal Calcium Dynamics
  • 06:42Results: Microglial Dynamics and Visual Responses in a Dendritic Spine
  • 07:44Conclusion

Summary

Traduction automatique

Traduction automatique

Here, we describe a protocol combining adeno-associated virus injection with cranial window implantation for simultaneous imaging of microglial dynamics and neuronal activity in awake mice.

Tags

Keywords: Simultaneous ImagingMicroglia DynamicsNeuronal ActivityAwake MiceAAV InjectionCranial WindowStereotaxic InstrumentMotion ArtifactPrimary Visual CortexMicroinjector

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