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Chapitres
- 00:04Introduction
- 00:53Isolation of Mouse Kidney Glomerular Cells
- 06:08Isolating the Glomerular Endothelial Cells with CD31‐Coated Beads
- 09:04Results: Isolation and Characterization of MitoDendra2‐GECs
- 11:04Conclusion
The article describes the method for isolating conditionally immortalized glomerular endothelial cells from the kidneys of transgenic mice expressing the thermolabile simian virus 40 and photo-activatable mitochondria, PhAMexcised. We describe the procedure for glomeruli isolation from whole kidneys using beads, digestion steps, seeding, and culturing of GECs-CD31 positive.
