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Biologie du développement
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Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

Zygote Microinjection for Creating Gene Cassette Knock-in and Flox Alleles in Mice

DOI:
10.3791/64161-v

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08:48 min

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June 24, 2022

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, , , , , , ,
1Laboratory Animal Resource Center and Trans-Border Medical Research Center,University of Tsukuba, 2Ph.D. Program in Human Biology, School of Integrative and Global Majors,University of Tsukuba

Chapitres

  • 00:04Introduction
  • 01:20Obtaining Mice Zygotes by Natural Mating
  • 02:29Preparation of the Microinjection Needle and Zygote Microinjection
  • 06:46Embryo Transfer into the Oviduct
  • 07:15Results: Analysis of Production Efficiency of Microinjection
  • 08:16Conclusion

Summary

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Traduction automatique

The present protocol describes zygote microinjection of CRISPR-Cas9 and donor DNA to efficiently produce gene cassette knock-in and floxed mice.

Tags

Zygote MicroinjectionGene Cassette Knock-inFlox AllelesGenetically-modified MiceIn Vivo ExperimentsGene ExpressionGenetic HumanizationConditional Gene KnockoutNeurologyImmunologyMetabolismReproductionInfectious DiseaseM2 MediumHyaluronidaseM16 MediumMicroinjection NeedleMicroforgeMicromanipulatorPolyvinyl Pyrrolidone

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