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Dissekering af celle-autonom funktion af skrøbeligt X mentalt retarderingsprotein i et auditivt kredsløb ved In Ovo-elektroporation
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Journal JoVE Neurosciences
Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

Dissekering af celle-autonom funktion af skrøbeligt X mentalt retarderingsprotein i et auditivt kredsløb ved In Ovo-elektroporation

DOI:
10.3791/64187-v

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11:10 min

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July 06, 2022

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, , ,
1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine,Jinan University, 2Department of Clinical Pathology,First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine,Florida State University

Chapitres

  • 00:05Introduction
  • 00:50Egg Preparation
  • 02:30Egg Windowing and Embryo Identification
  • 03:39Neural Tube Injection and Electroporation
  • 06:20Otocyst Injection and Electroporation
  • 07:44Results: FMRP Knockdown in the Chicken NM and Auditory Duct and Axonal Tracking of Transfected AG Neurons in the Brainstem
  • 10:18Conclusion

Summary

Traduction automatique

Traduction automatique

Ved hjælp af ovo elektroporation udtænkte vi en metode til selektivt at transficere det auditive indre øre og cochlearkernen i kyllingembryoner for at opnå en cellegruppespecifik knockdown af skrøbeligt X mentalt retarderingsprotein i diskrete perioder med kredsløbssamling.

Tags

Keywords: In Ovo ElectroporationCell-autonomous FunctionFMRPFragile X SyndromeAuditory CircuitStage-specificSite-specificDirection-specificFmr1 KnockdownDrug-inducible VectorEmbryo ManipulationHH StageAlbumen RemovalWindowingInk Injection

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