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Dissekere celle-autonom funksjon av skjøre X mental retardasjon protein i en auditiv krets ved i ovo elektroporering
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Journal JoVE Neurosciences
Dissecting Cell-Autonomous Function of Fragile X Mental Retardation Protein in an Auditory Circuit by In Ovo Electroporation

Dissekere celle-autonom funksjon av skjøre X mental retardasjon protein i en auditiv krets ved i ovo elektroporering

DOI:
10.3791/64187-v

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11:10 min

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July 06, 2022

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, , ,
1Division of Histology & Embryology, Key Laboratory for Regenerative Medicine of the Ministry of Education, College of Medicine,Jinan University, 2Department of Clinical Pathology,First Affiliated Hospital of Jinan University, 3Program in Neuroscience, Department of Biomedical Sciences, College of Medicine,Florida State University

Chapitres

  • 00:05Introduction
  • 00:50Egg Preparation
  • 02:30Egg Windowing and Embryo Identification
  • 03:39Neural Tube Injection and Electroporation
  • 06:20Otocyst Injection and Electroporation
  • 07:44Results: FMRP Knockdown in the Chicken NM and Auditory Duct and Axonal Tracking of Transfected AG Neurons in the Brainstem
  • 10:18Conclusion

Summary

Traduction automatique

Traduction automatique

Ved hjelp av ovo-elektroporering utviklet vi en metode for selektivt å transfektere det hørbare indre øret og cochleakjernen i kyllingembryoer for å oppnå en cellegruppespesifikk knockdown av skjørt X-mentalt retardasjonsprotein i diskrete perioder med kretsmontering.

Tags

Keywords: In Ovo ElectroporationCell-autonomous FunctionFMRPFragile X SyndromeAuditory CircuitStage-specificSite-specificDirection-specificFmr1 KnockdownDrug-inducible VectorEmbryo ManipulationHH StageAlbumen RemovalWindowingInk Injection

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