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鉴定甲型流感病毒感染期间切割蛋白质的半胱天冬酶及其基序
Journal JoVE
Immunologie et infection
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Journal JoVE Immunologie et infection
Identifying Caspases and their Motifs that Cleave Proteins During Influenza A Virus Infection
DOI:

08:16 min

July 21, 2022

Chapitres

  • 00:04Introduction
  • 00:45Assessing the Cleavage of Proteins by Caspases in IAV-Infected Cells
  • 02:55Confirmation of Caspase-Mediated Cleavage of Proteins in IAV-Infected Cells by RNA Interference
  • 04:13Site-Directed Mutagenesis for Locating the Caspase Cleavage Sites in the Polypeptide
  • 05:49Results: Role of Caspases in IAV Infection-Induced Degradation of Cortactin
  • 07:32Conclusion

Summary

Traduction automatique

甲型流感病毒(IAV)感染激活半胱天冬酶,切割宿主和病毒蛋白,而宿主和病毒蛋白又具有促病毒和抗病毒功能。通过使用抑制剂、RNA干扰、定点诱变以及蛋白质印迹和RT-qPCR技术,鉴定了受感染哺乳动物细胞中切割宿主皮质肌动蛋白和组蛋白脱乙酰酶的半胱天冬酶。

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