Journal
/
Recherche en cancérologie
/
Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells
Journal JoVE
Recherche en cancérologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Recherche en cancérologie
Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

Preparation of Cytoplasmic and Nuclear Long RNAs from Primary and Cultured Cells

DOI:
10.3791/64199-v

•

06:29 min

•

April 07, 2023

•

, , , , ,
1Sylvester Comprehensive Cancer Center,University of Miami Miller School of Medicine

Chapitres

  • 00:04Introduction
  • 01:11Separation of Nuclear and Cytoplasmic Fractions
  • 01:56Validation of Nuclear-Cytoplasmic Separation
  • 04:35Results: Confirmation of RNA Purity of Cytoplasmic and Nuclear Fraction
  • 05:48Conclusion

Summary

Traduction automatique

Traduction automatique

The present protocol offers an efficient and flexible method to isolate RNA from nuclear and cytoplasmic fractions using cultured cells, and then validate using qPCR. This effectively serves as a replacement for other RNA preparation kits.

Tags

Keywords: Cytoplasmic RNANuclear RNACell FractionationLysis BufferQPCRMALAT1TUG1Cell CultureIntracellular InteractionsNuclear-cytoplasmic ExchangeCancer Development

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/64199?article_type=v

Capture and Release of Viable Circulating Tumor Cells from Blood
check_url/fr/64199?article_type=v

Preparation and Metabolic Assay of 3-dimensional Spheroid Co-cultures of Pancreatic Cancer Cells and Fibroblasts
check_url/fr/64199?article_type=v

Development of a Human Preclinical Model of Osteoclastogenesis from Peripheral Blood Monocytes Co-cultured with Breast Cancer Cell Lines
check_url/fr/64199?article_type=v

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation
check_url/fr/64199?article_type=v

Characterization of Tumor Cells Using a Medical Wire for Capturing Circulating Tumor Cells: A 3D Approach Based on Immunofluorescence and DNA FISH
check_url/fr/64199?article_type=v

An Orthotopic Endometrial Cancer Model with Retroperitoneal Lymphadenopathy Made From In Vivo Propagated and Cultured VX2 Cells
check_url/fr/64199?article_type=v

Extraction of Aqueous Metabolites from Cultured Adherent Cells for Metabolomic Analysis by Capillary Electrophoresis-Mass Spectrometry
check_url/fr/64199?article_type=v

Subcellular Fractionation of Primary Chronic Lymphocytic Leukemia Cells to Monitor Nuclear/Cytoplasmic Protein Trafficking
check_url/fr/64199?article_type=v

Immunofluorescence Imaging of DNA Damage and Repair Foci in Human Colon Cancer Cells
check_url/fr/64199?article_type=v

Measurement of the Compressibility of Cell and Nucleus Based on Acoustofluidic Microdevice

Read Article