Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Laura Mosteo; Joana Reis; L&#237;dia Rocha; Marta Lopes; Delfim Duarte

doi:10.3791/64248

Journal

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Biologie

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Flowcytometrianalyse af murinknoglemarv, hæmatopoietisk stam- og stamceller og stromale nicheceller

Journal JoVE
Biologie

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Journal JoVE Biologie

Flow Cytometry Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells

Flowcytometrianalyse af murinknoglemarv, hæmatopoietisk stam- og stamceller og stromale nicheceller

DOI:

10.3791/64248-v

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08:34 min

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September 28, 2022

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Laura Mosteo, Joana Reis, Lídia Rocha, Marta Lopes, Delfim Duarte^2,3,4

¹Instituto de Investigação e Inovação em Saúde (i3S),Universidade do Porto, ²Department of Onco-Hematology,Instituto Português de Oncologia (IPO)-Porto, ³Unit of Biochemistry, Department of Biomedicine,Faculdade de Medicina da Universidade do Porto, ⁴Porto Comprehensive Cancer Center (P.CCC)

Chapitres

00:04Introduction
00:43Sample Extraction
01:47Sample Processing for Analysis of Hematopoietic Cell Populations in Total Bone Marrow
03:19Sample Processing for the Analysis of Stromal Cell Populations in Total Bone Marrow
05:13Sample Processing for the Analysis of Hematopoietic Cell Populations in Crushed and Flushed Bone Marrow
06:28Results: Phenotypic Analysis of Murine Bone Marrow Hematopoietic Stem and Progenitor Cells and Stromal Niche Cells by Flow Cytometry
07:40Conclusion

Summary

Traduction automatique

Her beskriver vi en simpel protokol til isolering og farvning af murinknoglemarvsceller til fænotype hæmopoietiske stam- og stamceller sammen med de understøttende nicheendotel- og mesenkymale stamceller. En metode til at berige celler placeret i endosteale og centrale knoglemarvsområder er også inkluderet.