Journal
/
Bio-ingénierie
/
Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation
Journal JoVE
Bio-ingénierie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Bio-ingénierie
Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

Experimental Quantification of Interactions Between Drug Delivery Systems and Cells In Vitro: A Guide for Preclinical Nanomedicine Evaluation

DOI:
10.3791/64259-v

•

08:47 min

•

September 28, 2022

•

, , , ,
1Department of Microbiology and Immunology,The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 2Department of Infectious Diseases,The University of Melbourne at the Peter Doherty Institute for Infection and Immunity, 3Victorian Infectious Diseases Service,Royal Melbourne Hospital at the Peter Doherty Institute for Infection and Immunity, 4Department of Infectious Diseases,Alfred Hospital and Monash University, 5Department of Chemical Engineering,The University of Melbourne, 6Department of Biomedical Engineering,The University of Melbourne

Chapitres

  • 00:05Introduction
  • 00:45Preparation
  • 01:58Measurement
  • 03:53Analysis
  • 05:11Determination of the Absolute Fluorescence Intensity per Carrier
  • 05:56Cell Experiment
  • 07:08Results: A Two-Stream Methodology to Convert Relative, Semiquantitative Flow Cytometry Cell-Carrier Association Data into Absolute Quantification
  • 08:17Conclusion

Summary

Traduction automatique

Traduction automatique

A workflow is demonstrated for the absolute quantification of drug carrier-cell interactions using flow cytometry to allow better rational evaluation of novel drug delivery systems. This workflow is applicable to drug carriers of any type.

Tags

Keywords: Quantitative AnalysisDrug Delivery SystemsCellsIn VitroNanoparticle Tracking AnalysisCarrier SystemsPreclinical Nanomedicine EvaluationCarrier PerformanceLinear RangeQuantitative RangeFlow CellCarrier DebrisCarrier SuspensionCarrier DilutionCarrier Concentration

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/64259?article_type=v

Models and Methods to Evaluate Transport of Drug Delivery Systems Across Cellular Barriers
check_url/fr/64259?article_type=v

Eye Irritation Test (EIT) for Hazard Identification of Eye Irritating Chemicals using Reconstructed Human Cornea-like Epithelial (RhCE) Tissue Model
check_url/fr/64259?article_type=v

Adapting the Electrospinning Process to Provide Three Unique Environments for a Tri-layered In Vitro Model of the Airway Wall
check_url/fr/64259?article_type=v

Sandwich-like Microenvironments to Harness Cell/Material Interactions
check_url/fr/64259?article_type=v

Synthesis of Functionalized 10-nm Polymer-coated Gold Particles for Endothelium Targeting and Drug Delivery
check_url/fr/64259?article_type=v

Comprehensive Evaluation of the Effectiveness and Safety of Placenta-Targeted Drug Delivery Using Three Complementary Methods
check_url/fr/64259?article_type=v

Use of High-Throughput Automated Microbioreactor System for Production of Model IgG1 in CHO Cells
check_url/fr/64259?article_type=v

Purification and Analytics of a Monoclonal Antibody from Chinese Hamster Ovary Cells Using an Automated Microbioreactor System
check_url/fr/64259?article_type=v

A Quantitative Fluorescence Microscopy-based Single Liposome Assay for Detecting the Compositional Inhomogeneity Between Individual Liposomes
check_url/fr/64259?article_type=v

Preparing Protein Producing Synthetic Cells using Cell Free Bacterial Extracts, Liposomes and Emulsion Transfer

Read Article