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Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Journal JoVE
Biologie

This content is Free Access.

Journal JoVE Biologie

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

Standardized Processing for Formalin-Fixed, Paraffin-Embedded Cell Pellet Immunohistochemistry Controls

DOI:

10.3791/64276-v

•

06:43 min

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July 27, 2022

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Charles Havnar, Kathy Hotzel, Carmina Espiritu, Amy Lo, Joshua D. Webster

¹Department of Pathology,Genentech

Chapitres

00:05Introduction
00:57Fixation of Cell Pellet
01:31Trimming and Processing of the Cell Pellet
03:23Embedding of Cell Pellet
04:27Sectioning of the Cell Pellet
05:01Results: Generating Formalin-Fixed, Paraffin-Embedded Cell Pellet Controls for Immunohistochemistry
05:53Conclusion

Summary

Traduction automatique

Presented here is a protocol for generating formalin-fixed, paraffin-embedded cell pellet controls for immunohistochemistry.

Tags

Cell Pellet Immunohistochemistry Controls Formalin-fixed Paraffin-embedded Hydroxyethyl Agarose Fixation Centrifugation Biomarker Development IHC Assay

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