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Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body
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Journal JoVE Biologie
Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body

Analyzing Starvation-Induced Autophagy in the Drosophila melanogaster Larval Fat Body

DOI:
10.3791/64282-v

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06:02 min

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August 04, 2022

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1Ministry of Education Key Laboratory of Biosystems Homeostasis and Protection and Innovation Center for Cell Signaling Network, Life Sciences Institute,Zhejiang University

Chapitres

  • 00:04Introduction
  • 00:40Drosophila Crossing and Egg Laying
  • 01:54Amino Acid Starvation Induces Autophagy
  • 02:51Third Instar Larvae Dissection and Sample Tissue Processing
  • 04:29Results: The Patterns of GFP‐Atg8a Puncta in Control, Mu1, and Mu2 Clones
  • 05:22Conclusion

Summary

Traduction automatique

Traduction automatique

The present protocol describes the induction of autophagy in the Drosophila melanogaster larval fat body via nutrient depletion and analyzes changes in autophagy using transgenic fly strains.

Tags

AutophagyDrosophila MelanogasterLarval Fat BodyClonal AnalysisAmino Acid DepletionThird Instar Larvae20% Sucrose SolutionDissection

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