/
/
Alerjik Kontakt Dermatitin Murin Modeli Olarak Kontakt Aşırı Duyarlılık
Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.
1Department of Clinical Immunology,Wroclaw Medical University, 2Department of Medical Physiology, Chair of Biomedical Sciences, Institute of Physiotherapy, Faculty of Health Sciences,Jagiellonian University Medical College, 3Chair of Biomedical Sciences, Faculty of Health Sciences,Jagiellonian University Medical College
Chapitres
- 00:04Introduction
- 00:59Marking Mice for Identification, and Induction of Contact Hypersensitivity (CHS)
- 02:19Ear Biopsies for Ear Weight and Myeloperoxidase (MPO) Assay
- 03:48Vascular Permeability Test
- 04:43Serum Collection and Anti-TNP Immunoglobulin (IgG1) Antibody Measurement
- 05:16Adoptive Transfer of the CHS-Effector Cells
- 06:41Results: Study of the Contact Hypersensitivity Reaction in Mice
- 07:42Conclusion
Kontakt aşırı duyarlılık (CHS), alerjik kontakt dermatitin (AKB) murin deneysel bir modelidir. CHS, göğüs ve karın bölgesinin traş edilmiş cildini boyayarak reaktif hapten ile hassaslaşmaya dayanır, daha sonra seyreltilmiş bir hapten ile kulak derisi meydan okuması ile çeşitli şekillerde değerlendirilen bir şişlik reaksiyonuna neden olur.
