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定量肽对白色念珠菌的抗真菌活性
Journal JoVE
Biologie
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Journal JoVE Biologie
Quantifying the Antifungal Activity of Peptides Against Candida albicans
DOI:

06:45 min

January 13, 2023

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Chapitres

  • 00:04Introduction
  • 00:51Inoculation, Culturing, and Subculturing of C. albicans
  • 01:29Preparation of the Peptide Solutions in a 96-Well Plate
  • 02:33Dilution of the C. albicans Subculture
  • 03:37Incubation of C. albicans</i with Peptide Solutions and Preparation of the Cells for the Quantification of Viability
  • 05:00Determination of Antifungal Activity
  • 05:34Results: Assessment of the Antifungal Activity of Histatin 5 and Variants of Histatin 5 Containing Modifications at the Lysine Residues
  • 06:05Conclusion

Summary

Traduction automatique

该协议描述了一种获取肽和其他化合物(例如小分子抗真菌剂)抗真菌活性的定量数据的方法,用于对抗 白色念珠菌。它使用光密度而不是计数集落形成单位来量化生长抑制,从而节省时间和资源。

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