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Chapitres
- 00:00Introduction
- 00:42Preparation of Plant Material
- 01:14Agroinfiltration
- 03:19Collection of Samples and Total Protein Extraction from Leaf
- 04:18Equilibrating the Desalting Column
- 04:49Magnetic Beads Washing and Enrichment of Biotinylated Proteins
- 06:56Results: AirID-Based Proximity Labeling in Cucumis sativus
- 07:50Conclusion
Here, we present a step-by-step protocol for performing the proximity labeling (PL) experiment in cucumber (Cucumis sativus L.) using AT4G18020 (APRR2)-AirID protein as a model. The method describes the construction of a vector, the transformation of a construct through agroinfiltration, biotin infiltration, protein extraction, and purification of biotin-labeled proteins through affinity purification technique.
