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小鼠卵母细胞质微管组织中心的多光子激光消融
Journal JoVE
Biologie du développement
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Journal JoVE Biologie du développement
Multi-Photon Laser Ablation of Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
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小鼠卵母细胞质微管组织中心的多光子激光消融

DOI:
10.3791/64439-v

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08:24 min

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November 11, 2022

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, ,
1Animal Sciences Research Center,University of Missouri, 2Universidad Nacional de Colombia, 3Advanced Light Microscopy Core,University of Missouri

Chapitres

  • 00:05Introduction
  • 01:12Mouse Oocyte Collection
  • 02:53Oocyte Microinjection
  • 04:16Oocyte Maturation
  • 05:02Microscope Preparation for Ablation
  • 06:05Ablation of Metaphase Cytoplasmic Microtubule Organizing Centers (mcMTOCs)
  • 06:59Results: Depletion of Metaphase Cytoplasmic Microtubule Organizing Centers in Mouse Oocytes
  • 07:35Conclusion

Summary

Traduction automatique

Traduction automatique

提出了一种优化的方案，该方案能够使用近红外飞秒激光在中期I期间消耗小鼠卵母细胞中的细胞质微管组织中心。

Tags

Multi-photon Laser AblationCytoplasmic Microtubule Organizing CentersMouse OocytesSpindle PositioningMeiosisAneuploidyMTOCsPolar MTOCsMcMTOCsTargeted AblationLive CellPhototoxicityMechanical PerturbationCell Viability

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