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Modellering af menneskelig cerebellær udvikling in vitro i 2D-struktur
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Journal JoVE Neurosciences
Modeling Human Cerebellar Development In Vitro in 2D Structure

Modellering af menneskelig cerebellær udvikling in vitro i 2D-struktur

DOI:
10.3791/64462-v

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06:14 min

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September 16, 2022

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, , , , ,
1Department of Psychiatry,University of Iowa, 2Department of Radiology,University of Iowa, 3Carver College of Medicine,University of Iowa, 4Iowa Neuroscience Institute,University of Iowa, 5Pappajohn Biomedical Institute,University of Iowa

Chapitres

  • 00:04Introduction
  • 00:41Experimental Preparation
  • 01:15Cerebellar Differentiation
  • 03:59Results: Overview of the 3D to 2D Cerebellar Differentiation and Expression of Cerebellar Cell Markers
  • 05:48Conclusion

Summary

Traduction automatique

Traduction automatique

Den nuværende protokol forklarer dannelsen af et 2D-monolag af cerebellære celler fra inducerede pluripotente stamceller til undersøgelse af de tidlige stadier af cerebellær udvikling.

Tags

Keywords: 2D StructureIn VitroCerebellar DevelopmentHumanNeuropsychiatric DisordersCost-efficientPulled Glass PipetteIPSCEmbryoid BodiesCerebellar Differentiation MediumFGF2

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