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基于靶向质谱的低丰度蛋白质蛋白分析中的微量采样
Journal JoVE
Chimie
This content is Free Access.
Journal JoVE Chimie
Microsampling in Targeted Mass Spectrometry-Based Protein Analysis of Low-Abundance Proteins
DOI:

10:21 min

January 13, 2023

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Chapitres

  • 00:05Introduction
  • 00:46Preparation of Antibody-Coated Magnetic Beads
  • 03:41Volumetric Absorptive Microsampling and Elution
  • 04:38Digestion of Eluted VAMS
  • 05:30The Capture of Proteotypic ProGRP Using Antibody-Coated Magnetic Beads
  • 07:37Analysis by LC-MS/MS
  • 08:26Results: Analysis of the MS Chromatograms and Base Peak Chromatograms of the Proteotypic Peptide
  • 09:42Conclusion

Summary

Traduction automatique

提出了一种方案,用于从干燥的血清样品中测定低丰度生物标志物,例如生物标志物前胃泌素释放肽(ProGRP)。抗体包被的磁珠用于蛋白型ProGRP肽的选择性纯化和富集。随后通过液相色谱-串联质谱分析捕获的肽。

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