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Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder
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Biochimie
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Journal JoVE Biochimie
Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder

Live-Cell Imaging of Intact Ex Vivo Globes Using a Novel 3D Printed Holder

DOI:
10.3791/64510-v

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05:41 min

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October 06, 2022

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, , , ,
1Department of Pharmacology, School of Medicine,Boston University, 2Department of Biochemistry, School of Medicine,Boston University, 3Department of Ophthalmology, School of Medicine,Boston University

Chapitres

  • 00:04Introduction
  • 00:48Sample Collection and Preparation of Sample Holders
  • 02:03Wounding of the Eyes
  • 02:54Sample Placement on the Holder and Sample Imaging
  • 04:18Results: High‐Resolution Live Cell Imaging of Enucleated Globes
  • 05:14Conclusion

Summary

Traduction automatique

Traduction automatique

The present work describes a novel experimental protocol that utilizes a 3D printed holder to enable high-resolution live cell imaging of enucleated globes. Through this protocol, the cellular calcium signaling activity in wounded corneal epithelium from ex vivo globes can be observed in real time.

Tags

Keywords: 3D Printed HolderLive-cell ImagingEx Vivo GlobesCorneal CellsEpitheliumNervesWound HealingCalcium IndicatorCell Membrane StainScratch WoundPuncture WoundCorneal Basement Membrane

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