Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

Journal JoVE
Recherche en cancérologie

Un abonnement à JoVE est nécessaire pour voir ce contenu. Connectez-vous ou commencez votre essai gratuit.

Journal JoVE Recherche en cancérologie

DOI:

10.3791/64535-v

•

06:08 min

•

February 10, 2023

•

Claudia Manriquez Roman^2,3,4,5, R. Leo Sakemura², Brooke L. Kimball², Fang Jin, Roman H. Khadka, Mohamad M. Adada², Elizabeth L. Siegler², Aaron J. Johnson, Saad S. Kenderian^2,6

¹T Cell Engineering Laboratory,Mayo Clinic, Rochester, ²Division of Hematology,Mayo Clinic, Rochester, ³Mayo Clinic Graduate School of Biomedical Sciences,Mayo Clinic, Rochester, ⁴Department of Molecular Medicine,Mayo Clinic, Rochester, ⁵Regenerative Sciences PhD Program,Mayo Clinic, Rochester, ⁶Department of Immunology,Mayo Clinic, Rochester

Chapitres

00:04Introduction
00:49Assessment of CART19 Cell‐Associated Toxicities
01:33MRI Imaging
04:02Results: Assessment of CART Cell‐Associated Toxicities in a PDX Mice Model
05:17Conclusion

Summary

Traduction automatique

Here, we describe a protocol in which an acute lymphoblastic leukemia patient-derived xenograft model is used as a strategy to assess and monitor CD19-targeted chimeric antigen receptor T cell-associated toxicities.

Assessment of Chimeric Antigen Receptor T Cell-Associated Toxicities Using an Acute Lymphoblastic Leukemia Patient-Derived Xenograft Mouse Model

•

•

•

Chapitres

Summary

Traduction automatique

Tags

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

Development and Maintenance of a Preclinical Patient Derived Tumor Xenograft Model for the Investigation of Novel Anti-Cancer Therapies

Preparation of Primary Acute Lymphoblastic Leukemia Cells in Different Cell Cycle Phases by Centrifugal Elutriation

Flow Cytometry to Estimate Leukemia Stem Cells in Primary Acute Myeloid Leukemia and in Patient-derived-xenografts, at Diagnosis and Follow Up

Modeling Osteosarcoma Using Li-Fraumeni Syndrome Patient-derived Induced Pluripotent Stem Cells

Assessment of the Metabolic Profile of Primary Leukemia Cells

Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis

In Vitro Tumor Cell Rechallenge For Predictive Evaluation of Chimeric Antigen Receptor T Cell Antitumor Function

Patient-derived Heterogeneous Xenograft Model of Pancreatic Cancer Using Zebrafish Larvae as Hosts for Comparative Drug Assessment

A Melanoma Patient-Derived Xenograft Model

Establishment of Gastric Cancer Patient-derived Xenograft Models and Primary Cell Lines

Read Article