/
/
Engineering onkogen heterozygot gain-of-function mutationer i humane hæmatopoietiske stamceller og stamceller
This content is Free Access.
1Division of Hematology, Department of Internal Medicine,Medical University of Graz, 2Division of Hematology, Stanford Cancer Institute, Stanford Institute for Stem Cell Biology and Regenerative Medicine,Stanford University School of Medicine, 3Department of Blood Group Serology and Transfusion Medicine,Medical University of Graz
Chapitres
- 00:05Introduction
- 00:54sgRNA Design
- 01:57Homology-Directed Repair Vector Construction
- 05:42Editing of Hematopoietic Stem and Progenitor Cells
- 09:48Results: Heterozygous Gain-of-Function Mutations in Hematopoietic Stem and Progenitor Cells by Combining the Use of CRISPR/Cas9 and Dual rAAV Donor Transduction
- 11:19Conclusion
Nye strategier til trofast modellering af somatiske mutationer i hæmatopoietiske stam- og stamceller (HSPC'er) er nødvendige for bedre at studere hæmatopoietisk stamcellebiologi og hæmatologiske maligniteter. Her beskrives en protokol til modellering af heterozygote gain-of-function-mutationer i HSPC'er ved at kombinere brugen af CRISPR/Cas9 og dual rAAV donortransduktion.
