Journal
/
Recherche en cancérologie
/
Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs
Journal JoVE
Recherche en cancérologie
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Recherche en cancérologie
Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs

Establishing 3-Dimensional Spheroids from Patient-Derived Tumor Samples and Evaluating their Sensitivity to Drugs

DOI:
10.3791/64564-v

•

10:38 min

•

December 16, 2022

•

, , , , , , ,
1Felsenstein Medical Research Center, 2Davidoff Center, Rabin Medical Center, 3BioInsight Ltd., 4Sackler Faculty of Medicine,Tel Aviv University

Chapitres

  • 00:04Introduction
  • 01:05Generation and Collection of Spheroids
  • 05:24Drug Efficacy Assay (MTT Assay)
  • 08:05Results: Formation of Spheroids From a Primary Colon Cancer Cell Culture, the Effects of Treatments on Spheroids, and the Follow-Up Over Time
  • 10:04Conclusion

Summary

Traduction automatique

Traduction automatique

The present protocol describes generating 3D tumor culture models from primary cancer cells and evaluating their sensitivity to drugs using cell-viability assays and microscopic examinations.

Tags

3D Tumor ModelPatient-derived Tumor Samples3D SpheroidsDrug SensitivityPersonalized Cancer TreatmentPrimary Cancer CellsCell CultureAccutase3D Culture MediumUltra-low Attachment Plate

Article

Embed

AJOUTER À LA PLAYLIST

Usage Statistics

Vidéos Connexes

check_url/fr/64564?article_type=v

Labeling of Breast Cancer Patient-derived Xenografts with Traceable Reporters for Tumor Growth and Metastasis Studies
check_url/fr/64564?article_type=v

Isolation and Characterization of Tumor-initiating Cells from Sarcoma Patient-derived Xenografts
check_url/fr/64564?article_type=v

High-sensitivity Detection of Micrometastases Generated by GFP Lentivirus-transduced Organoids Cultured from a Patient-derived Colon Tumor
check_url/fr/64564?article_type=v

Mimicking and Manipulating Pancreatic Acinar-to-Ductal Metaplasia in 3-dimensional Cell Culture
check_url/fr/64564?article_type=v

Patient-derived Orthotopic Xenograft Models for Human Urothelial Cell Carcinoma and Colorectal Cancer Tumor Growth and Spontaneous Metastasis
check_url/fr/64564?article_type=v

Physiologic Patient Derived 3D Spheroids for Anti-neoplastic Drug Screening to Target Cancer Stem Cells
check_url/fr/64564?article_type=v

Establishment and Characterization of Small Bowel Neuroendocrine Tumor Spheroids
check_url/fr/64564?article_type=v

Isolation of Stem-like Cells from 3-Dimensional Spheroid Cultures
check_url/fr/64564?article_type=v

Drug Screening of Primary Patient Derived Tumor Xenografts in Zebrafish
check_url/fr/64564?article_type=v

High-Throughput In Vitro Assay using Patient-Derived Tumor Organoids

Read Article