Journal
/
/
부유 기반 T 세포 분리, 활성화 및 마이크로버블을 사용한 인간 말초 혈액 단핵 세포 샘플로부터의 확장
Journal JoVE
Immunologie et infection
Un abonnement à JoVE est nécessaire pour voir ce contenu.  Connectez-vous ou commencez votre essai gratuit.
Journal JoVE Immunologie et infection
Flotation-Based T Cell Isolation, Activation, and Expansion from Human Peripheral Blood Mononuclear Cell Samples Using Microbubbles
DOI:

06:37 min

December 23, 2022

, , ,

Chapitres

  • 00:04Introduction
  • 00:29Isolation of T Cells with Microbubbles Using Positive Selection
  • 01:51Co-Stimulation (Activation) of the Positively Selected T Cells
  • 02:52Expansion of the Co-Stimulated Cells in Cell Culture Medium
  • 03:28Expansion of the T Cells (With or Without Prior Transduction)
  • 04:02Harvesting the T Cells and Flow Cytometry
  • 05:16Results: Assessment of Viable and Successfully Transduced Cells
  • 05:58Conclusion

Summary

Traduction automatique

이 연구의 목적은 일차 인간 T 세포를 분리, 활성화 및 확장하기 위한 부유 기반 분리의 타당성을 입증하는 것입니다.

Vidéos Connexes

Read Article