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A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse
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Biologie du développement
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Journal JoVE Biologie du développement
A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

A Pipeline to Characterize Structural Heart Defects in the Fetal Mouse

DOI:
10.3791/64582-v

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08:19 min

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December 16, 2022

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, , , , , , , ,
1Department of Developmental Biology,University of Pittsburgh, 2Department of Biological Sciences,University of Pittsburgh, 3Département de Biologie,École Normale Supérieure de Lyon, 4Department of Critical Care Medicine,University of Pittsburgh

Chapitres

  • 00:05Introduction
  • 01:05Fetal Echocardiogram
  • 01:46Necropsy
  • 02:41Episcopic Confocal Microscopy (ECM)
  • 04:01Three-Dimensional (3D) Reconstruction
  • 06:14Results: Analyzing Cardiac Anomalies Using Echo and ECM
  • 07:19Conclusion

Summary

Traduction automatique

Traduction automatique

This article details murine congenital heart disease (CHD) diagnostic methods using fetal echocardiography, necropsy, and Episcopic fluorescence image capture (EFIC) using Episcopic confocal microscopy (ECM) followed by three-dimensional (3D) reconstruction.

Tags

Keywords: Fetal MouseCongenital Heart DefectsEchocardiographyNecropsyConfocal MicroscopyAnatomical ChangesStructural Birth DefectsUltrasoundColor DopplerSample PreparationLaser Confocal ImagingImage Acquisition

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