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Chapitres
- 00:05Introduction
- 01:03Preparation of the Multi‐Well Device
- 03:00Preparation of the Multi‐Well Device for Media Loading
- 03:29Filling the Wells with Low Melting Nematode Growth Media (lmNGM)
- 05:53Adding Copper Sulfate to the Moat and Adding Autoclaved Water Crystals
- 06:55Spotting the Wells with Concentrated Bacteria
- 07:29Adding Worms to the Multi‐Well Device and Finishing the Preparation of the Device for Long‐Term Use
- 08:00Results: Correlation of Measured Phenotypes Across Populations in Individual Animals
- 08:55Conclusion
Presented here is an optimized protocol for culturing isolated individual nematodes on solid media in microfabricated multi-well devices. This approach allows individual animals to be monitored throughout their lives for a variety of phenotypes related to aging and health, including activity, body size and shape, movement geometry, and survival.
