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Chapitres
- 00:05Introduction
- 00:49Prepare Palmitic Acid Working Solution
- 01:27Applying Palmitic Acid Coating to Microtray
- 02:53Loading the Microtray with Low‐Melt Nematode Growth Media (lmNGM) and Seeding the Microtray Wells with Bacterial Food
- 03:47Enclosing Each Microtray Inside a Single‐Well Plate
- 04:25Adding Worms to the Microtray and Preparing the Culture Environment for Long‐Term Use
- 05:01Imaging Individual Worms in Microtray Wells
- 05:27Results: Correlation of Phenotypes in Individual Animals Across Populations
- 06:53Conclusion
Her præsenterer vi en protokol til dyrkning af isolerede individuelle nematoder på faste medier til livslang fysiologisk parametersporing og fluorescenskvantificering. Dette kultursystem omfatter en palmitinsyrebarriere omkring enkeltormbrønde for at forhindre dyr i at flygte, hvilket tillader anvendelse af aversive indgreb, herunder patogene bakterier og kemiske stressorer.
