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Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran
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Biologie
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Journal JoVE Biologie
Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran

Assessment of Gut Barrier Integrity in Mice Using Fluorescein-Isothiocyanate-Labeled Dextran

DOI:
10.3791/64710-v

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05:14 min

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November 18, 2022

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, , ,
1Nutrition and Microbiome Laboratory, Institut du cancer de Montréal,Centre de recherche du Centre hospitalier de l’Université de Montréal (CRCHUM), 2Digestive Surgery Service, Centre hospitalier de l’Université de Montréal (CHUM), Faculty of Medicine,Université de Montréal, 3Department of Medicine, Faculty of Medicine,Université de Montréal

Chapitres

  • 00:05Introduction
  • 00:39Administration of FITC-Dextran
  • 01:09In Vivo Fluorescence Measurement
  • 02:44Fluorescence Measurement in Fecal Samples and Plasma
  • 03:50Results: Dietary Supplementation with Inulin and the Translocation of FITC-Dextran
  • 04:45Conclusion

Summary

Traduction automatique

Traduction automatique

In the present study, fluorescein-isothiocyanate-labeled (FITC) dextran is administered to mice via oral gavage to evaluate intestinal permeability both in vivo and in plasma and fecal samples. As gut barrier function is affected in many disease processes, this direct and quantitative assay can be used in diverse research areas.

Tags

Keywords: FITC-dextranGut Barrier IntegrityIntestinal PermeabilityIn VivoNon-invasiveOral GavageFluorescence ImagingFecalPlasma

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