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Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization
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Journal JoVE Génétique
Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization

Exploring X Chromosomal Aberrations in Ovarian Cells by Using Fluorescence In Situ Hybridization

DOI:
10.3791/64734-v

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11:08 min

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April 07, 2023

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, , , ,
1Department of Obstetrics and Gynecology,Radboudumc, 2Department of Pediatrics,Radboudumc, Amalia Children’s Hospital, 3Department of Reproductive Medicine,Nij Geertgen Center for Fertility

Chapitres

  • 00:05Introduction
  • 00:52Fluorescence In Situ Hybridization (FISH) on Isolated Individual Ovarian Cortex Cells
  • 05:55FISH on Paraffin Sections of Grafted Ovarian Cortex Tissue
  • 08:20Results: Determining X Chromosomal Content of Ovarian Cells in Non-Grafted and Grafted Ovarian Cortex Tissue
  • 10:23Conclusion

Summary

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This article presents two methods based on fluorescence in situ hybridization to determine the X chromosomal content of ovarian cells in non-grafted and grafted ovarian cortex tissue from females with X chromosomal aberrations.

Tags

X Chromosomal AberrationsOvarian CellsFluorescence In Situ Hybridization (FISH)Stromal CellsFolliclesTrypsin/EDTA/glucosePotassium ChlorideDPBSMethanol-acetic Acid Fixation

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