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Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Journal JoVE
Biochimie
Author Produced

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Journal JoVE Biochimie

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

Measuring Caspase Activity Using a Fluorometric Assay or Flow Cytometry

DOI:

10.3791/64745-v

•

05:29 min

•

March 24, 2023

•

Jing Tong, Stefanie Rufli, Wendy Wei-Lynn Wong

¹Institute of Experimental Immunology,University of Zürich

Chapitres

00:04Introduction
00:45Seeding, Cell Treatment, and Preparation of Cell Lysate
02:10BCA Assay for Protein Quantification
02:49Population-Based Assay for Caspase-3/7 Activity
03:31Single-Cell Assay for Caspase-3/7 Activity
04:07Results: Smac Mimetic-Induced Effect on Caspase-3/7 Activity
04:50Conclusion

Summary

Traduction automatique

The present protocol describes two methods to measure caspase activity through a fluorogenic substrate using flow cytometry or a spectrofluorometer.

Tags

Keywords: Caspase Activity Fluorometric Assay Flow Cytometry Cell Death SMAC Mimetic Bone Marrow-derived Macrophages (BMDMs)DISC Lysis Buffer BCA Assay Protein Quantification Fluorometric Instrument

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