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Multiplexed Barcoding Image Analysis for Immunoprofiling and Spatial Mapping Characterization in the Single-Cell Analysis of Paraffin Tissue Samples
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Chapitres
- 00:04Introduction
- 01:01Oligonucleotide‐Conjugated Antibody Staining
- 04:39Multiplexed Imaging Reporter Plate
- 05:16Operating the Multiplexed Imaging Machine
- 06:31Multiplex Image Collection and Analysis
- 07:01Results: Visualization of the Acquired Multiplexed Images
- 07:28Conclusion
Multiplexed barcoding image analysis has recently improved the characterization of the tumor microenvironment, permitting comprehensive studies of cell composition, functional state, and cell-cell interactions. Herein, we describe a staining and imaging protocol using the barcoding of oligonucleotide-conjugated antibodies and cycle imaging, which allows for the use of a high-dimensional image analysis technique.
