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Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis
Journal JoVE
Biologie
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Journal JoVE Biologie
Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis

Efficient and Consistent Generation of Retinal Pigment Epithelium/Choroid Flatmounts from Human Eyes for Histological Analysis

DOI:
10.3791/64761-v

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07:59 min

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October 28, 2022

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, , , ,
1Ocular and Stem Cell Translational Research Section, National Eye Institute,National Institutes of Health (NIH)

Chapitres

  • 00:04Introduction
  • 00:46Silicone Mold Preparation
  • 01:21RPE Dissection
  • 03:42Staining
  • 04:45ReShAPE Analysis
  • 06:17Results: Complete Morphometric Analysis of Entire Human RPE Monolayer
  • 07:21Conclusion

Summary

Traduction automatique

Traduction automatique

We describe a method to efficiently separate retinal pigment epithelium (RPE) from the retina in human eyes and generate whole RPE/choroid flatmounts for histological and morphometric analyses of the RPE.

Tags

Retinal Pigment EpitheliumRPEChoroidFlatmountHistological AnalysisRetinal DegenerationDissection TechniqueREShAPE SoftwareD-MannitolDPBSPFAMacular RegionCiliary Margin

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