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Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
Journal JoVE
Biologie
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Journal JoVE Biologie
Cell Culture Techniques and Practices to Avoid Contamination by Fungi and Bacteria in the Research Cell Culture Laboratory
DOI:

13:39 min

July 07, 2023

Chapitres

  • 00:04Introduction
  • 00:47Preparations
  • 01:59Inside the Biological Safety Cabinet
  • 04:16Working with Adherent Cells
  • 06:13Checking and Storing Cells
  • 07:19Working with Suspension Cells
  • 08:52Cell Incubation
  • 09:05Liquid Waste Collection
  • 09:29Clean Up
  • 10:38Organization
  • 11:13Representative Results
  • 12:42Conclusion

Summary

Traduction automatique

This protocol presents essential cell culture techniques and practices to be used in the research cell culture laboratory to avoid contamination by fungi and bacteria. Within the category of bacteria, special emphasis will be placed on preventing mycoplasma contamination.  

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